Rapid High-resolution Brain Mapping with CLARITY Optimized Light Sheet Microscopy (COLM)

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Rapid 3D light-sheet microscopy with a tunable lens.

The in-vivo investigation of highly dynamic biological samples, for example the beating zebrafish heart, requires high-speed volume imaging techniques. Light-sheet microscopy is ideal for such samples as it records high-contrast images of entire planes within large samples at once. However, in order to obtain images of different planes, it has been necessary to move the sample relative to the f...

متن کامل

Internet-enabled high-resolution brain mapping and virtual microscopy.

Virtual microscopy involves the conversion of histological sections mounted on glass microscope slides to high-resolution digital images. Virtual microscopy offers several advantages over traditional microscopy, including remote viewing and data sharing, annotation, and various forms of data mining. We describe a method utilizing virtual microscopy for generation of internet-enabled, high-resol...

متن کامل

High-Resolution Light-Field Microscopy

By combining a high-resolution image from a standard camera with a low-resolution wavefront measurement from a Shack-Hartmann sensor, we numerically reconstruct a highresolution light field. We experimentally demonstrate the method with a commercially available microscope. ©2013 Optical Society of America OCIS codes: (110.0180) Microscopy; (110.1758) Computational imaging; (100.6640) Superresol...

متن کامل

Hyperspectral light sheet microscopy

To study the development and interactions of cells and tissues, multiple fluorescent markers need to be imaged efficiently in a single living organism. Instead of acquiring individual colours sequentially with filters, we created a platform based on line-scanning light sheet microscopy to record the entire spectrum for each pixel in a three-dimensional volume. We evaluated data sets with varyin...

متن کامل

Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution.

Although fluorescence microscopy provides a crucial window into the physiology of living specimens, many biological processes are too fragile, are too small, or occur too rapidly to see clearly with existing tools. We crafted ultrathin light sheets from two-dimensional optical lattices that allowed us to image three-dimensional (3D) dynamics for hundreds of volumes, often at subsecond intervals...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: Microscopy and Microanalysis

سال: 2015

ISSN: 1431-9276,1435-8115

DOI: 10.1017/s1431927615004389